prepGEM Universal

MicroGEM’s prepGEM Universal is a widely-used, adaptable reagent kit suitable for DNA extractions from life science samples. Because the entire extraction is done in a single-tube with no washes or additional steps, there is no loss of DNA, enabling extractions from even a single cell.

• Extracts DNA from blood, saliva, and tissue including animal (ear punches, mouse tails, insect) and cell culture
• Produces single-stranded DNA for STR, PCR, qPCR, whole genome amplification, amplicon sequencing
• Perfect for CRISPR/Cas9 genotyping workflows
• Each extraction kit contains prepGEM, Histosolv, BLUE buffer, RED+ buffer, and ORANGE+ buffer

Overview:

prepGEM Universal is MicroGEM’s comprehensive solution for effortless high-yield DNA extraction from blood, saliva, and tissues. It is also perfectly suited for extracting DNA from cell cultures. All within a single tube, this method ensures no loss of DNA, and as a result, it excels with low cell number extractions.

This kit is an excellent tool for researchers performing confirmatory genotyping of transfected cells following CRISPR/Cas9 gene editing.

prepGEM Universal utilizes our thermophilic proteinase by activation with a simple temperature change. The whole process is easily automated in a single tube using standard laboratory equipment. A standard thermocycler can be programmed to carry out the extraction.

• Stream-lined approach provides DNA in approximately 15 minutes
• Inhibitor-free solutions mean no harsh chemical washes or multiple steps are required
• Closed-tube extraction protects the integrity of the sample
• Simplified, hands-off workflow frees up technician time, reduces lab costs, and significantly reduces hazardous/plastic waste